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Anti-Nrf2 antibody (ab31163)抗核轉(zhuǎn)錄因子2抗體產(chǎn)品信息：
- Product nameAnti-Nrf2 antibodySee all Nrf2 primary antibodies ...
- Description
Rabbit polyclonal to Nrf2
- Specificityab31163 recognises Nrf2 protein
- Tested applicationsIHC-P, ICC/IF, IHC-Fr, WB more details
- Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Cow - Immunogen
Synthetic peptide: TL YLEVFSMLRD EDGKPYSP, corresponding to amino acids 569-588 of Human Nrf2
- Positive control
- Placenta
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.1% Sodium Azide
Constituents: 1% BSA, 10mM PBS, pH 7.4 Concentration 500 µl at 0.2 mg/ml
- PurityProtein A purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
- Epigenetics and Nuclear Signaling
- Transcription
- Other factors
- Cell Biology
- Other Antibodies
- Oxidative Stress
- Cardiovascular
- Heart
- Cardiac metabolism
- Metabolism
- Pathways and Processes
- Mitochondrial Metabolism
- Mitochondrial Biogenesis
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Nucleotide metabolism
- Molecular processes
- Mitochondrial transcription
- Metabolism
- Pathways and Processes
- Redox metabolism
- Oxidative stress
Applications
Our Abpromise guarantee covers the use of ab31163 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| IHC-P | IHC-P: 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
| ICC/IF | ICC/IF: Use at an assay dependent dilution. |
| IHC-Fr | IHC-Fr: Use at an assay dependent dilution. PubMed: 17142801 |
| WB | WB: 1/1000. Detects a band of approximay 68 kDa (predicted molecular weight: 68 kDa). See Abreview. |
Target
- FunctionTranscription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.
- Tissue specificityWidely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle.
- Sequence similaritiesBelongs to the bZIP family. CNC subfamily.
Contains 1 bZIP domain. - DomainAcidic activation domain in the N-terminus, and DNA binding domain in the C-terminus.
- Post-translational
modificationsPhosphorylation of Ser-40 by PKC in response to oxidative stress dissociates NFE2L2 from its cytoplasmic inhibitor KEAP1, promoting its translocation into the nucleus. - Cellular localizationCytoplasm > cytosol. Nucleus. Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electrophilic agents.
- Information by UniProt
Database links
- Entrez Gene: 396014 Chicken
- Entrez Gene: 497024 Cow
- Entrez Gene: 4780 Human
- Entrez Gene: 18024 Mouse
- Entrez Gene: 83619 Rat
- Omim: 600492 Human
- SwissProt: Q5NUA6 Cow
- SwissProt: Q16236 Human
Alternative names
- erythroid derived 2 antibody
- HEBP1 antibody
- like 2 antibody
Anti-Nrf2 antibody images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nrf2 antibody (ab31163)
ab31163 at 1/100 dilution, staining Nrf2 in human placenta by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections).
Immunocytochemistry/ Immunofluorescence-Nrf2 antibody(ab31163)
ICC/IF image of ab31163 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab31163, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - Nrf2 antibody (ab31163)
All lanes : Anti-Nrf2 antibody (ab31163) at 1 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : A498 (Human Kidney Carcinoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 68 kDa
Observed band size : 68 kDa
Additional bands at : 28 kDa,37 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 1 minute
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